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cd34 multisort kit  (Miltenyi Biotec)


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    Miltenyi Biotec cd34 multisort kit
    Cd34 Multisort Kit, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 95 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd34+multisort+kit/CD34+MultiSort+Kit%2C+human/pmc12408697-57-22-27
    Average 93 stars, based on 95 article reviews
    cd34 multisort kit - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Isolation:

    Article Title: Deciphering the Functional Long Non‐Coding RNAs Derived from MicroRNA Loci
    Article Snippet: .. After isolation by HISTOPAQUE (Sigma‐Aldrich, Deisenhofen, Germany) density gradient centrifugation, CD34 + cells were enriched from Mononuclear cells through positive immunomagnetic selection (CD34 MultiSort kit, Miltenyi Biotec, Bergisch‐Glad‐bach, Germany). ..

    Article Title: Total ginsenosides enhance γ-globin expression and fetal hemoglobin production in β-thalassemia models
    Article Snippet: .. The mononuclear cell fraction was isolated using LymphoprepTM (STEMCELL Technologies, Canada), and subsequent purification of CD34 + cells was performed using the CD34 MultiSort Kit for humans (Miltenyi Biotec, Germany) for monocyte sorting ( ). ..

    Article Title: Non-Viral Episomal Vector Mediates Efficient Gene Transfer of the β-Globin Gene into K562 and Human Haematopoietic Progenitor Cells.
    Article Snippet: .. CD34+ cells were isolated from low-density mononuclear cells (MNC) derived from cord blood by density gradient centrifugation (1.077 g/mL Ficoll-Paque, Biochrom, Cambridge, UK) and immunomagnetic selection, using a combination of the Miltenyi CD34 MultiSort kit (Miltenyi Biotec, Hong Kong, China) and the LS Columns (Miltenyi Biotec), in accordance with the manufacturer’s instructions. ..

    Article Title: Non-Viral Episomal Vector Mediates Efficient Gene Transfer of the β-Globin Gene into K562 and Human Haematopoietic Progenitor Cells
    Article Snippet: .. CD34+ cells were isolated from low-density mononuclear cells (MNC) derived from cord blood by density gradient centrifugation (1.077 g/mL Ficoll-Paque, Biochrom, Cambridge, UK) and immunomagnetic selection, using a combination of the Miltenyi CD34 MultiSort kit (Miltenyi Biotec, Hong Kong, China) and the LS Columns (Miltenyi Biotec), in accordance with the manufacturer’s instructions. ..

    Article Title: Deciphering the Functional Long Non-Coding RNAs Derived from MicroRNA Loci.
    Article Snippet: .. After isolation by HISTOPAQUE (Sigma-Aldrich, Deisenhofen, Germany) density gradient centrifugation, CD34+ cells were enriched from Mononuclear cells through positive immunomagnetic selection (CD34 MultiSort kit, Miltenyi Biotec, Bergisch-Glad-bach, Germany). ..

    Article Title: Proteomics screening uncovers HMGA1 as a promising negative regulator for γ-globin expression in response to decreased β-globin levels.
    Article Snippet: Reactivation of fetal hemoglobin (HbF) is a critical goal for the treatment of patients with hemoglobinopathies. β-globin disorders can trigger stress erythropoiesis in red blood cells (RBCs).. Cell-intrinsic erythroid stress signals promote erythroid precursors to express high levels of fetal hemoglobin, which is also known as γ-globin.. However, the molecular mechanism underlying γ-globin production during cell-intrinsic erythroid stress remains to be elucidated.

    Article Title: Galectin-1: An important regulator in myeloid differentiation and acute myeloid leukemia as well as a promising prognostic indicator and therapeutic target.
    Article Snippet: Acute myeloid leukemia (AML) is an aggressive and heterogeneous hematological malignancy with a low survival probability and limited therapeutic options.. Although galectin-1 (LGALS1) has been implicated in tumor cell survival and immune evasion in solid tumor, its role in AML is still unclear.. In this study, we found that LGALS1 presents prominent upregulation in AML patients at both mRNA and protein levels compared with the control samples.

    Gradient Centrifugation:

    Article Title: Deciphering the Functional Long Non‐Coding RNAs Derived from MicroRNA Loci
    Article Snippet: .. After isolation by HISTOPAQUE (Sigma‐Aldrich, Deisenhofen, Germany) density gradient centrifugation, CD34 + cells were enriched from Mononuclear cells through positive immunomagnetic selection (CD34 MultiSort kit, Miltenyi Biotec, Bergisch‐Glad‐bach, Germany). ..

    Article Title: Non-Viral Episomal Vector Mediates Efficient Gene Transfer of the β-Globin Gene into K562 and Human Haematopoietic Progenitor Cells.
    Article Snippet: .. CD34+ cells were isolated from low-density mononuclear cells (MNC) derived from cord blood by density gradient centrifugation (1.077 g/mL Ficoll-Paque, Biochrom, Cambridge, UK) and immunomagnetic selection, using a combination of the Miltenyi CD34 MultiSort kit (Miltenyi Biotec, Hong Kong, China) and the LS Columns (Miltenyi Biotec), in accordance with the manufacturer’s instructions. ..

    Article Title: Non-Viral Episomal Vector Mediates Efficient Gene Transfer of the β-Globin Gene into K562 and Human Haematopoietic Progenitor Cells
    Article Snippet: .. CD34+ cells were isolated from low-density mononuclear cells (MNC) derived from cord blood by density gradient centrifugation (1.077 g/mL Ficoll-Paque, Biochrom, Cambridge, UK) and immunomagnetic selection, using a combination of the Miltenyi CD34 MultiSort kit (Miltenyi Biotec, Hong Kong, China) and the LS Columns (Miltenyi Biotec), in accordance with the manufacturer’s instructions. ..

    Article Title: Deciphering the Functional Long Non-Coding RNAs Derived from MicroRNA Loci.
    Article Snippet: .. After isolation by HISTOPAQUE (Sigma-Aldrich, Deisenhofen, Germany) density gradient centrifugation, CD34+ cells were enriched from Mononuclear cells through positive immunomagnetic selection (CD34 MultiSort kit, Miltenyi Biotec, Bergisch-Glad-bach, Germany). ..

    Selection:

    Article Title: Deciphering the Functional Long Non‐Coding RNAs Derived from MicroRNA Loci
    Article Snippet: .. After isolation by HISTOPAQUE (Sigma‐Aldrich, Deisenhofen, Germany) density gradient centrifugation, CD34 + cells were enriched from Mononuclear cells through positive immunomagnetic selection (CD34 MultiSort kit, Miltenyi Biotec, Bergisch‐Glad‐bach, Germany). ..

    Article Title: Non-Viral Episomal Vector Mediates Efficient Gene Transfer of the β-Globin Gene into K562 and Human Haematopoietic Progenitor Cells.
    Article Snippet: .. CD34+ cells were isolated from low-density mononuclear cells (MNC) derived from cord blood by density gradient centrifugation (1.077 g/mL Ficoll-Paque, Biochrom, Cambridge, UK) and immunomagnetic selection, using a combination of the Miltenyi CD34 MultiSort kit (Miltenyi Biotec, Hong Kong, China) and the LS Columns (Miltenyi Biotec), in accordance with the manufacturer’s instructions. ..

    Article Title: Non-Viral Episomal Vector Mediates Efficient Gene Transfer of the β-Globin Gene into K562 and Human Haematopoietic Progenitor Cells
    Article Snippet: .. CD34+ cells were isolated from low-density mononuclear cells (MNC) derived from cord blood by density gradient centrifugation (1.077 g/mL Ficoll-Paque, Biochrom, Cambridge, UK) and immunomagnetic selection, using a combination of the Miltenyi CD34 MultiSort kit (Miltenyi Biotec, Hong Kong, China) and the LS Columns (Miltenyi Biotec), in accordance with the manufacturer’s instructions. ..

    Article Title: Deciphering the Functional Long Non-Coding RNAs Derived from MicroRNA Loci.
    Article Snippet: .. After isolation by HISTOPAQUE (Sigma-Aldrich, Deisenhofen, Germany) density gradient centrifugation, CD34+ cells were enriched from Mononuclear cells through positive immunomagnetic selection (CD34 MultiSort kit, Miltenyi Biotec, Bergisch-Glad-bach, Germany). ..

    Article Title: Proteomics screening uncovers HMGA1 as a promising negative regulator for γ-globin expression in response to decreased β-globin levels.
    Article Snippet: Reactivation of fetal hemoglobin (HbF) is a critical goal for the treatment of patients with hemoglobinopathies. β-globin disorders can trigger stress erythropoiesis in red blood cells (RBCs).. Cell-intrinsic erythroid stress signals promote erythroid precursors to express high levels of fetal hemoglobin, which is also known as γ-globin.. However, the molecular mechanism underlying γ-globin production during cell-intrinsic erythroid stress remains to be elucidated.

    Article Title: Galectin-1: An important regulator in myeloid differentiation and acute myeloid leukemia as well as a promising prognostic indicator and therapeutic target.
    Article Snippet: Acute myeloid leukemia (AML) is an aggressive and heterogeneous hematological malignancy with a low survival probability and limited therapeutic options.. Although galectin-1 (LGALS1) has been implicated in tumor cell survival and immune evasion in solid tumor, its role in AML is still unclear.. In this study, we found that LGALS1 presents prominent upregulation in AML patients at both mRNA and protein levels compared with the control samples.

    Purification:

    Article Title: Total ginsenosides enhance γ-globin expression and fetal hemoglobin production in β-thalassemia models
    Article Snippet: .. The mononuclear cell fraction was isolated using LymphoprepTM (STEMCELL Technologies, Canada), and subsequent purification of CD34 + cells was performed using the CD34 MultiSort Kit for humans (Miltenyi Biotec, Germany) for monocyte sorting ( ). ..

    Derivative Assay:

    Article Title: Non-Viral Episomal Vector Mediates Efficient Gene Transfer of the β-Globin Gene into K562 and Human Haematopoietic Progenitor Cells.
    Article Snippet: .. CD34+ cells were isolated from low-density mononuclear cells (MNC) derived from cord blood by density gradient centrifugation (1.077 g/mL Ficoll-Paque, Biochrom, Cambridge, UK) and immunomagnetic selection, using a combination of the Miltenyi CD34 MultiSort kit (Miltenyi Biotec, Hong Kong, China) and the LS Columns (Miltenyi Biotec), in accordance with the manufacturer’s instructions. ..

    Article Title: Non-Viral Episomal Vector Mediates Efficient Gene Transfer of the β-Globin Gene into K562 and Human Haematopoietic Progenitor Cells
    Article Snippet: .. CD34+ cells were isolated from low-density mononuclear cells (MNC) derived from cord blood by density gradient centrifugation (1.077 g/mL Ficoll-Paque, Biochrom, Cambridge, UK) and immunomagnetic selection, using a combination of the Miltenyi CD34 MultiSort kit (Miltenyi Biotec, Hong Kong, China) and the LS Columns (Miltenyi Biotec), in accordance with the manufacturer’s instructions. ..

    Article Title: Galectin-1: An important regulator in myeloid differentiation and acute myeloid leukemia as well as a promising prognostic indicator and therapeutic target.
    Article Snippet: Acute myeloid leukemia (AML) is an aggressive and heterogeneous hematological malignancy with a low survival probability and limited therapeutic options.. Although galectin-1 (LGALS1) has been implicated in tumor cell survival and immune evasion in solid tumor, its role in AML is still unclear.. In this study, we found that LGALS1 presents prominent upregulation in AML patients at both mRNA and protein levels compared with the control samples.

    Cell Isolation:

    Article Title: Proteomics screening uncovers HMGA1 as a promising negative regulator for γ-globin expression in response to decreased β-globin levels.
    Article Snippet: Reactivation of fetal hemoglobin (HbF) is a critical goal for the treatment of patients with hemoglobinopathies. β-globin disorders can trigger stress erythropoiesis in red blood cells (RBCs).. Cell-intrinsic erythroid stress signals promote erythroid precursors to express high levels of fetal hemoglobin, which is also known as γ-globin.. However, the molecular mechanism underlying γ-globin production during cell-intrinsic erythroid stress remains to be elucidated.



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    Figure 2. Comparative immunopeptidome profiling identifies AML/LSC-associated HLA class I antigen targets. A, Saturation analysis of HLA class I-restricted peptide source proteins of the AML cohort. The mean number of unique source proteins (y-axis) for a given cohort size (number of samples, x-axis) has been calculated by 1,000 random samplings from the entirety of AML immunopeptidomes. The number of unique source protein identifica- tions (y-axis) is shown as a function of cumulative immunopeptidome analysis of AML samples (n = 47, x-axis). Exponential regression was used to extrapolate the maximum attainable coverage of different source proteins (dotted line, 11,193 proteins). The dashed line depicts the HLA-restricted peptide source proteome coverage achieved in the AML cohort (10,906 proteins). B, HLA class I allotype population coverage within the AML cohort com- pared with the world population (www.iedb.org). The frequencies of individuals within the world population carrying up to six HLA allotypes (x-axis) of the AML dataset are indicated as gray bars on the left y-axis. The cumulative percentage of population coverage is depicted as black dots on the right y-axis. C, Overlap analysis of HLA class I ligand identifications of primary AML samples (n = 47, curated immunopeptidome data) and benign samples (n = 332). D, Allotype-specific comparative immunopeptidome profiling based on the frequency of HLA-A*01–restricted peptide presentation in HLA-A*01–posi- tive AML (n = 14) and benign samples (n = 93). Frequencies of positive immunopeptidomes for the respective HLA ligand (x-axis) are indicated on the y-axis. HLA-A*01 ligands (n = 7,346) are depicted on the x-axis, sorted according to the frequency of AML and benign samples presenting the respective ligand. The box on the left highlights the subset of AML-associated antigens showing AML-exclusive, high frequent presentation. E, Overlap analysis of HLA class I ligand identifications of LSC samples (n = 10) with AML bulk (n = 47) and benign samples including <t>CD34+-enriched</t> HSPCs (n = 332). F, Pro- portion of all HLA class I and of HLA class I allotype-specific high frequent AML-associated peptides that are also presented on LSCs (AML/LSC shared antigens). N indicates the number of peptides. Abbreviations: ID, identification; IPep, immunopeptidome.
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    Figure 2. Comparative immunopeptidome profiling identifies AML/LSC-associated HLA class I antigen targets. A, Saturation analysis of HLA class I-restricted peptide source proteins of the AML cohort. The mean number of unique source proteins (y-axis) for a given cohort size (number of samples, x-axis) has been calculated by 1,000 random samplings from the entirety of AML immunopeptidomes. The number of unique source protein identifica- tions (y-axis) is shown as a function of cumulative immunopeptidome analysis of AML samples (n = 47, x-axis). Exponential regression was used to extrapolate the maximum attainable coverage of different source proteins (dotted line, 11,193 proteins). The dashed line depicts the HLA-restricted peptide source proteome coverage achieved in the AML cohort (10,906 proteins). B, HLA class I allotype population coverage within the AML cohort com- pared with the world population (www.iedb.org). The frequencies of individuals within the world population carrying up to six HLA allotypes (x-axis) of the AML dataset are indicated as gray bars on the left y-axis. The cumulative percentage of population coverage is depicted as black dots on the right y-axis. C, Overlap analysis of HLA class I ligand identifications of primary AML samples (n = 47, curated immunopeptidome data) and benign samples (n = 332). D, Allotype-specific comparative immunopeptidome profiling based on the frequency of HLA-A*01–restricted peptide presentation in HLA-A*01–posi- tive AML (n = 14) and benign samples (n = 93). Frequencies of positive immunopeptidomes for the respective HLA ligand (x-axis) are indicated on the y-axis. HLA-A*01 ligands (n = 7,346) are depicted on the x-axis, sorted according to the frequency of AML and benign samples presenting the respective ligand. The box on the left highlights the subset of AML-associated antigens showing AML-exclusive, high frequent presentation. E, Overlap analysis of HLA class I ligand identifications of LSC samples (n = 10) with AML bulk (n = 47) and benign samples including <t>CD34+-enriched</t> HSPCs (n = 332). F, Pro- portion of all HLA class I and of HLA class I allotype-specific high frequent AML-associated peptides that are also presented on LSCs (AML/LSC shared antigens). N indicates the number of peptides. Abbreviations: ID, identification; IPep, immunopeptidome.
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    Image Search Results


    Figure 2. Comparative immunopeptidome profiling identifies AML/LSC-associated HLA class I antigen targets. A, Saturation analysis of HLA class I-restricted peptide source proteins of the AML cohort. The mean number of unique source proteins (y-axis) for a given cohort size (number of samples, x-axis) has been calculated by 1,000 random samplings from the entirety of AML immunopeptidomes. The number of unique source protein identifica- tions (y-axis) is shown as a function of cumulative immunopeptidome analysis of AML samples (n = 47, x-axis). Exponential regression was used to extrapolate the maximum attainable coverage of different source proteins (dotted line, 11,193 proteins). The dashed line depicts the HLA-restricted peptide source proteome coverage achieved in the AML cohort (10,906 proteins). B, HLA class I allotype population coverage within the AML cohort com- pared with the world population (www.iedb.org). The frequencies of individuals within the world population carrying up to six HLA allotypes (x-axis) of the AML dataset are indicated as gray bars on the left y-axis. The cumulative percentage of population coverage is depicted as black dots on the right y-axis. C, Overlap analysis of HLA class I ligand identifications of primary AML samples (n = 47, curated immunopeptidome data) and benign samples (n = 332). D, Allotype-specific comparative immunopeptidome profiling based on the frequency of HLA-A*01–restricted peptide presentation in HLA-A*01–posi- tive AML (n = 14) and benign samples (n = 93). Frequencies of positive immunopeptidomes for the respective HLA ligand (x-axis) are indicated on the y-axis. HLA-A*01 ligands (n = 7,346) are depicted on the x-axis, sorted according to the frequency of AML and benign samples presenting the respective ligand. The box on the left highlights the subset of AML-associated antigens showing AML-exclusive, high frequent presentation. E, Overlap analysis of HLA class I ligand identifications of LSC samples (n = 10) with AML bulk (n = 47) and benign samples including CD34+-enriched HSPCs (n = 332). F, Pro- portion of all HLA class I and of HLA class I allotype-specific high frequent AML-associated peptides that are also presented on LSCs (AML/LSC shared antigens). N indicates the number of peptides. Abbreviations: ID, identification; IPep, immunopeptidome.

    Journal: Blood Cancer Discovery

    Article Title: Immune Surveillance of Acute Myeloid Leukemia Is Mediated by HLA-Presented Antigens on Leukemia Progenitor Cells

    doi: 10.1158/2643-3230.bcd-23-0020

    Figure Lengend Snippet: Figure 2. Comparative immunopeptidome profiling identifies AML/LSC-associated HLA class I antigen targets. A, Saturation analysis of HLA class I-restricted peptide source proteins of the AML cohort. The mean number of unique source proteins (y-axis) for a given cohort size (number of samples, x-axis) has been calculated by 1,000 random samplings from the entirety of AML immunopeptidomes. The number of unique source protein identifica- tions (y-axis) is shown as a function of cumulative immunopeptidome analysis of AML samples (n = 47, x-axis). Exponential regression was used to extrapolate the maximum attainable coverage of different source proteins (dotted line, 11,193 proteins). The dashed line depicts the HLA-restricted peptide source proteome coverage achieved in the AML cohort (10,906 proteins). B, HLA class I allotype population coverage within the AML cohort com- pared with the world population (www.iedb.org). The frequencies of individuals within the world population carrying up to six HLA allotypes (x-axis) of the AML dataset are indicated as gray bars on the left y-axis. The cumulative percentage of population coverage is depicted as black dots on the right y-axis. C, Overlap analysis of HLA class I ligand identifications of primary AML samples (n = 47, curated immunopeptidome data) and benign samples (n = 332). D, Allotype-specific comparative immunopeptidome profiling based on the frequency of HLA-A*01–restricted peptide presentation in HLA-A*01–posi- tive AML (n = 14) and benign samples (n = 93). Frequencies of positive immunopeptidomes for the respective HLA ligand (x-axis) are indicated on the y-axis. HLA-A*01 ligands (n = 7,346) are depicted on the x-axis, sorted according to the frequency of AML and benign samples presenting the respective ligand. The box on the left highlights the subset of AML-associated antigens showing AML-exclusive, high frequent presentation. E, Overlap analysis of HLA class I ligand identifications of LSC samples (n = 10) with AML bulk (n = 47) and benign samples including CD34+-enriched HSPCs (n = 332). F, Pro- portion of all HLA class I and of HLA class I allotype-specific high frequent AML-associated peptides that are also presented on LSCs (AML/LSC shared antigens). N indicates the number of peptides. Abbreviations: ID, identification; IPep, immunopeptidome.

    Article Snippet: MACS was performed with the human CD34 MultiSort (Miltenyi Biotec, catalog no. 130–056–701) and CD38 MicroBead Kits (Miltenyi Biotec, catalog no. 130–092–263).

    Techniques: